Changes in version 0.2.4 (2026-02-27) Major Changes - New functions 'cyt_univariate' for t-test and wilcoxon rank sum test and 'cyt_univariate_multi' for ANOVA and Kruskal-Wallis test have been added to conduct univariate statistical analysis for multiple groups. - Changed 'cyt_bp' to adapt grouping similar to 'cyt_bp2' so there is no longer a need for separate functions - New function 'cyt_violin' for creating violin plots with statistical comparisons between groups. - Two new helper functions to apply different types of transformations and adjust p-values. - Added more options for scale/transformations in all functions that require it and added p-value adjustment options where needed. Changes in version 0.2.3 (2025-12-06) Major Changes - Make 'cyt_xgb' compatible with the upcoming XGBoost R package changes (parameter renames, changes in predict() output). - Fix ROC/AUC computation and confusion matrix computation for XGBoost models. - Deprecate arguments 'eta' and 'gamma' in cyt_xgb() in favor of 'learning_rate' and 'min_split_loss' using the lifecycle package. Changes in version 0.2.2 (2025-09-04) Major Changes - 'cyt_mint_splsda', a new function for conducting multivariate integrative analysis using sparse partial least squares discriminant analysis (sPLS-DA) with multiple datasets, has been added. - 'cyt_splsda' now has new arguments: - 'batch_col' to specify a batch column for batch correction in the sPLS-DA analysis using a Z-score method. - 'multilevel_col' to specify a multilevel column for multilevel analysis in sPLS-DA. - 'ind_names' to specify custom individual names for the samples in the plots. - Added new "ExampleData5" dataset which is the same as "ExampleData1" but with a batch column for demonstration of the 'batch_col' argument in 'cyt_mint_splsda'. - 'cyt_heatmap' now uses pheatmap for plotting, and provides a proper legend along with additional scaling options. Minor Changes and Bug Fixes - Updated the vignette and README files to include examples of the new 'cyt_mint_splsda' function. - Updated 'cyt_splsda' and 'cyt_pca' logic. Changes in version 0.2.1 (2025-05-19) Major Changes - 'cyt_ttest' now conducts shapiro-wilk test and based on the p-value decides to do Welch two sample t-test or Wilcoxon Rank Sum Test. Minor Changes and Bug Fixes - Updated 'cyt_pca' and 'cyt_splsda' to use the appropriate pch argument in plotIndiv(). - Updated 'cyt_ttest' examples. - Updated citation formatting. - Changed links Changes in version 0.2.0 (2025-04-10) Major Changes - 'cyt_splsda': Fixed an error occurring when only one variable has VIP > 1 leading to not being able to conduct sPLS-DA analysis. Now, the function checks whether the number of VIP > 1 variables is below 2, if that is true the sPLS-DA model of VIP > 1 is skipped. - Fixed an issue with the 'verbose' argument of 'cyt_splsda' not working in overall analysis due to calling the wrong object. - 'cyt_errbp' now uses 'ggplot2' with 'facet_wrap' to create multiple error-bar plots where the p-value and effect size labels are based on t-test comparisons between the groups. Minor Changes and Bug Fixes - 'cyt_errbp', 'cyt_bp', 'cyt_bp2', and 'cyt_skku' received a minor theme update keeping the background white in scenarios where it may appear transparent due to using 'theme_minimal()'. - Fixed some grammatical and spelling issues in the README file. - Vignette for the package now show plots generated that aren't saved to PDF files. A temporary directory is not created anymore. Changes in version 0.1.2 (2025-04-03) Major Changes - 'cyt_rf', 'cyt_splsda', 'cyt_ttest', 'cyt_dualflashplot', and 'cyt_volc' now have a logical 'verbose' argument to print the output if user wants to. This is to ensure printed output in console can be easily suppressed. - 'cyt_xgb' has a new logical argument called 'print_results' that works the same as the 'verbose' argument in other functions mentioned above. - Reverted the previous 'verbose' conditionals on plots from the functions mentioned above as they are essential to the output. For example, after creating a ggplot2 object of the plots, I have left 'print(a)' without a 'verbose' conditional statement. - Have removed the arguments for changing graphical parameters in 'cyt_bp', and 'cyt_bp2' so it no longer requires changes to the user's graphical parameters. - Added a new 'format_output' argument to 'cyt_anova' and 'cyt_ttest' to format the output as a data frame which can be printed to show neat format, however still dependent on whether 'verbose' equals TRUE or FALSE. - 'cyt_rf', 'cyt_splsda', and 'cyt_xgb' now has a 'seed' argument to set the seed for reproducibility. Minor Changes and Bug Fixes - Added references in description field of the DESCRIPTION file to the multivariate methods mentioned in the format 'Authors (Year) doi:...'. - Updated examples from graphical parameters changing without resetting properly to now the graphical parameters reverting to original using 'oldpar <- par(no.readonly = TRUE)' in the beginning and par(oldpar) after execution of code. - Updated 'getting_started.Rmd' vignette where the files created and saved are now saved to a temporary directory using 'tempdir()' to avoid creating files in the user's working directory. Additionally, the vignette now uses 'oldpar <- par(no.readonly = TRUE)' in the beginning and par(oldpar) after code execution to revert to original graphical parameters for the 'cyt_errbp' examples shown. Changes in version 0.1.1 - Fixed some general CRAN automated test issues. - Updated functions and examples to avoid generating PDF and PNG files during checks. - Functions now also generated plots within RStudio graphics device and provides the option to save the plots as PDF or PNG files depending on the function. Changes in version 0.1.0 - Initial CRAN submission. Since this is our initial submission, our NEWS.md contains a single entry indicating the initial release. We plan to provide more detailed change logs in subsequent versions. Changes in version 0.0.0.9000 - Preparation For Initial CRAN Submission This version is the development version of CytoProfile. We are preparing for the initial CRAN submission. Below are the features added in this version: Major Changes - Added functions for ANOVA, Boxplots, Dual-Flashlight Plot, Error-Bar Plot, Heatmap, Principal Component Analysis (PCA), Random Forest, Skewness and Kurtosis, Sparse Partial Least Squares - Discriminant Analysis (sPLS-DA), T-Test, Volcano Plot, and Extreme Gradient Boosting (XGBoost). Minor Changes and Bug Fixes - Added a vignette to demonstrate the usage of the package. - Added built-in data sets for demonstration purposes. - Examples from the documentation are now working as expected. - 'cyt_splsda' is now able to use one grouping column to run analysis. - 'cyt_skku' now prints the output in readable format.